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老年癡呆癥模式小鼠胃菌群結構的研究.doc

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  • 論文格式:Word格式(*.doc)
  • 更新時間:2014-07-12
  • 論文字數:11117
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摘要:人體內龐大的微生物群體對人體有著巨大的影響。本文基于此對老年癡呆癥的發生與胃腸道菌群的關系進行了研究。針對8例老年癡呆癥模式小鼠與8例健康小鼠的胃容物樣本進行預處理后,提取樣本菌群基因組DNA,并進行16S rRNA v3區片段PCR擴增,擴增產物用變性梯度凝膠電泳法(DGGE)檢測分析。結果發現,老年癡呆癥模式小鼠組與健康小鼠組的胃腸道菌群相似性指數僅為0.4左右。實驗結果對研究老年癡呆癥的發病機理及并發癥治療有重要意義,為預防老年癡呆癥的發生奠定理論基礎。

關鍵詞:胃腸道菌群;16S rRNA;PCR;變性梯度凝膠電泳

 

Abstract:The microbial communities great affect human physiology. Based on it,the relationship of the occurrence of Alzheimer's Disease and intestinal microbiota structure were investigated. In this study, stomach samples of 8 Alzheimer's Disease model mice and 8 healthy mice were collected. Total bacteria genomic DNA was extracted from the fecal samples after pre-treatment.For 16S r RNA V3 region PCR amplification,and PCR products were detected and analyzed by denaturing gradient gel electrophoresis(DGGE).Comparing the stomach content of Alzheimer's Disease model mice group and health mice groups,significant differences were found as shown in similarity index is only about 0.4. The experimental conclusion can be applied in the study of intestinal microbial diversity,which may be of significance for the research of diabetes pathogenesis, establish the theoretical basis for preventing Alzheimer's Disease.

Key words:stomach microbiota structure;16S r RNA;PCR;DGGE

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